We characterized 45 LMW glutenin genes from three diploid species of Taeniatherum using
63 primer combinations, designed according to 264 genes reported in
wheat and related species. The genes had 909–1,059 bp nucleotides and
301–351 amino acids. The deduced peptides shared similar structures with
LMW-m proteins of wheat. The 45 genes shared 77.2–99.7% identities in
peptide sequence among each other and 60.0–82.0% identities to proteins
from wheat and related species. They were divided into five types
according to the N-terminals, starting with METSCIP-, METSRVP-,
METGRIP-, METGSIP- and VETSCIP-. The last three and some other
structural domain variations were not reported previously in the
Triticeae. Thirty-three genes encoded full mature proteins with intact
ORFs, whereas the other 12 were pseudogenes with incomplete ORFs,
in-frame stop codons or frame-shift mutations. Phylogenetic analysis
showed that orthologous genes from Taeniatherum were more similar to those in the B and D genomes than in the A genome.
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