王际睿, 博士, 教授 Jirui Wang, Ph.D., Professor
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email： isphsc@sicau.edu.cn jirui.wang@gmail.com
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教育经历（Education）：
l 2004.9-2008.7 Ph.D. 作物遗传育种（Plant Breeding）, 四川农业大学（Sichuan Agricultural University）
l 2001.9-2004.7 MSc作物遗传育种（Plant Breeding）, 四川农业大学（Sichuan Agricultural University）
l 1997.9-2001.7 BSc 生化与分子生物学（Biochemistry & Molecular biology）, 兰州大学（Lanzhou University）
学术与职业经历（Academic & Professional Experience）：
l 四川农业大学小麦研究所（Triticeae Research Institute, Sichuan Agricultural University, Chengdu Sichuan, 2008-Now）. I am a PI in Triticeae Research Institute, SICAU. The main works in our group are 1) characterization of mechanisms for seed development and germination in cereals; 2) PHS resistant wheat breeding, and 3) wheat quality enhancement
l 加拿大农业部渥太华研究中心（ECORC-AAFC, Ottawa, ON, Canada, Jan 2007- Feb 2008）. A project (supported by the Chinese and Canadian government) about characterizing seed genes with specific expression levels in Fusarium infected wheat by microarray and Q-PCR was carried out.
l 美国加州大学戴维斯（UC DAVIS, USA, Apr 2011-Mar 2013）. I worked with Drs. Mingcheng Luo and Jan Dvorak during 2011-2013 as postdoc fellow. We researched the adaptive evolution of diploid wheat Ae.tauschii. Please see: “Aegilops tauschii single nucleotide polymorphisms shed light on the origins of wheat D-genome genetic diversity and pinpoint the geographic origin of hexaploid wheat” in New Phytologist 2013. This project was supported by NSF and USDA.
l 国际谷物穗发芽大会主委会委员/主席（Committee member of International Symposium of Pre-harvest sprouting on Cereals, 2017-Now）.
l 种子科学特邀编辑（Guest Editor of <Seed Science Research>, 2019-2021）.
目前研究及招生方向（Current Research）：
The goal of our group is to understand the environment affection, epigenetic mechanisms, and gene networks that control seed development, dormancy, and germination in cereals. The environment, epigenetic mechanisms, and gene networks affect many essential processes of seed, including embryo development, grain filling, maturation, longevity, and germination. Many of them are necessary for crop production, such as yield, quality, nutrition, and PHS resistance.
Pre-harvest sprouting (PHS) is defined as the germination of grains in the ears before harvest, which causes a decrease in end-use quality due to starch and protein degradation. PHS is considered a worldwide disaster that reduces the production of cereals and damages their quality. In recent years, our group is working on the characterization of PHS resistant genes and selecting elite breeding materials. More than 1000 germplasms were assayed for PHS resistance and used for gene mining. Three main QTL and 32 grain color-related QTL (GCR-QTL) were detected by GWAS. GCR-QTL occurred at high frequencies in the red-grained accessions, and a strong correlation was observed between the number of GCR-QTL and the germination ratio. Further, investigation of the origin of haplotypes associated with the three main QTL revealed that favorable haplotypes for PHS resistance were more common in accessions from higher rainfall zones. Moreover, we detected two primary PHS resistant genes from synthetic wheat that originated from Ae.tauschii and T.turgidum, respectively.
l 穗发芽及其对小麦品质的影响
对种子成熟到萌发过程中的调控基因进行分析，揭示穗发芽抗性与特定基因遗传变异的相关关系；明确控制小麦籽粒穗发芽与延缓品质劣化的关键基因，并分析其对穗发芽抗性程度的影响，解析抗穗发芽形成的分子机制。
l 种子发育与萌发的生物学调控
基于我们开发的转录因子、非编码RNA、甲基化芯片以及数据库，对小麦、高粱等种子发育-萌发相关基因进行分析，发掘决定种子发育与萌发的关键因子，通过遗传分析及CRISPR/Cas9基因编辑鉴定其功能，并构建各因子间互作网络，达到解析种子大小、粒色、萌发、寿命等表型特征机制的目的。
部分研究工作（Selected works）:
l Aegilops tauschii single nucleotide polymorphisms shed light on the origins of wheat D-genome genetic diversity and pinpoint the geographic origin of hexaploid wheat. New Phytologist 2013, 198: 925-937
Hexaploid wheat (Triticum aestivum, genomes AABBDD) originated by hybridization of tetraploid Triticum turgidum (genomes AABB) with Aegilops tauschii (genomes DD). Genetic relationships between A. tauschii and the wheat D genome are of central importance for the understanding of the wheat origin and subsequent evolution. Genetic relationships among 477 A. tauschii and wheat accessions were studied with the A. tauschii 10K Infinium single nucleotide polymorphism (SNP) array. Aegilops tauschii consists of two lineages (designated 1 and 2) having little genetic contact. Each lineage consists of two closely related sublineages. A population within lineage 2 in the southwestern and southern Caspian appears to be the main source of the wheat D genome. Lineage 1 contributed as little as 0.8% of the wheat D genome. Triticum aestivum is subdivided into the western and Far Eastern populations. The Far Eastern population conserved the genetic make-up of the nascent T. aestivum more than the western population. In wheat, diversity is high in chromosomes 1D and 2D, and it correlates in all wheat D-genome and A. tauschii chromosomes with recombination rates. Gene flow from A. tauschii was an important source of wheat genetic diversity and shaped its distribution along the D-genome chromosomes.
l Uncovering the dispersion history, adaptive evolution and selection of wheat in China. Plant Biotechnology Journal, 2018, 16: 280-291
Wheat was introduced to China approximately 4500 years ago, where it adapted over a span of time to various environments in agro-ecological growing zones. We investigated 717 Chinese and 14 Iranian/Turkish geographically diverse, locally adapted wheat landraces with 27,933 DArTseq (for 717 landraces) and 312,831 Wheat660K (for a subset of 285 landraces) markers. This study highlights the adaptive evolutionary history of wheat cultivation in China. Environmental stresses and independent selection efforts have resulted in considerable genome-wide divergence at the population level in Chinese wheat landraces. In total, 148 regions of the wheat genome show signs of selection in at least one geographic area. Our data show adaptive events across geographic areas, from the xeric northwest to the mesic south, along and among homoeologous chromosomes, with fewer variations in the D genome than in the A and B genomes. Multiple variations in interdependent functional genes, such as regulatory and metabolic genes controlling germination and flowering time were characterized, showing clear allelic frequency changes corresponding to the dispersion of wheat in China. Population structure and selection data reveal that Chinese wheat spread from the northwestern Caspian Sea region to south China, adapting during its agricultural trajectory to increasingly mesic and warm climatic areas.
l WheatGmap: A Comprehensive Platform for Wheat Gene Mapping and Genomic Studies. Molecular Plant, 2021, 14: 187-190
Gene mapping of mutations is a critical step for new gene discovery and functional analysis. However, this step has been limited by the discovery of very dense sets of informative markers. Here, we report a platform, Wheat Genomic Map (WheatGmap, https://www.wheatgmap.org), for the fast and cost-effective mapping of genes in wheat. The main application of this platform is the use of several statistical models enabling researchers to simply and flexibly conduct bulked segregant analysis (BSA)-based causal variation mining and gene mapping. A significant feature of the platform is that large-scale genomic variant data can be used as a resource to filter non-causal SNPs or non-validated candidate genes. Moreover, users can share their genomics data of genetic information and annotated phenotypes via WheatGmap. In the current version (WheatGmap 1.0), more than 3,500 next-generation sequencing datasets, including whole-exome sequencing (WES), whole-genome sequencing (WGS), and RNA-seq datasets from public resources, were processed with our standard pipeline. These online tools and genomics data could become an easily applicable resource for gene mapping in a complex wheat genome background.
l Myb10-D confers PHS-3D resistance to pre-harvest sprouting by regulating NCED in ABA biosynthesis pathway of wheat. New Phytologist 2021, Online
Pre-harvest sprouting (PHS), the germination of grain before harvest, is a serious problem resulting in wheat yield and quality losses. Here, we mapped the PHS resistance gene PHS-3D from synthetic hexaploid wheat to a 2.4 Mb presence-absence variation (PAV) region and found that its resistance effect was attributed to the pleiotropic Myb10-D by integrated omics and functional analyses. Three haplotypes were detected in this PAV region among 262 worldwide wheat lines and 16 Aegilops tauschii, and the germination percentages of wheat lines containing Myb10-D was approximately 40% lower than that of the other lines. Transcriptome and metabolome profiling indicated that Myb10-D affected the transcription of genes in both the flavonoid and ABA biosynthesis pathways, which resulted in increases in flavonoids and ABA in transgenic wheat lines. Myb10-D activates NCED by biding the secondary wall MYB-responsive element (SMRE) to promote ABA biosynthesis in early wheat seed development stages. We revealed that the newly discovered function of Myb10-D confers PHS resistance by enhancing ABA biosynthesis to delay germination in wheat. The PAV harboring Myb10-D associated with grain color and PHS will be useful for understanding and selecting white-grained PHS resistant wheat cultivars.
Lab News:
20210325: 肖春生等论文“Characterization and expression quantitative trait loci analysis of TaABI4, a pre-harvest sprouting related gene in wheat” 在Seed Science Research发表。
20210302: 郎静等论文“Myb10-D confers PHS-3D resistance to pre-harvest sprouting by regulating NCED in ABA biosynthesis pathway of wheat” 在New Phytologist发表。
20210203: 张立超、董春豪、陈中序等论文“WheatGmap: A Comprehensive Platform for Wheat Gene Mapping and Genomic Studies” 在Molecular Plant发表。
20201108: 符语昕等论文“Identification and Characterization of PLATZ Transcription Factors in Wheat” 在IJMS发表。
20190829：杨剑等论文“Identification of qPHS.sicau-1B and qPHS.sicau-3D from synthetic wheat for pre-harvest sprouting resistance wheat improvement”在Molecular Breeding发表。
20190730-0802：第十四届“国际谷物穗发芽大会”顺利召开（isphsc.csp.escience.cn）。
大会共吸引到了来自中国、美国、日本、澳大利亚、英国等国家80余名行业内专家学者到场，围绕“种子休眠与发芽”、“淀粉酶与穗发芽”、“穗发芽分子遗传机制”、“穗发芽抗性育种”四个专题展开了深入的交流与讨论。刘宇娇、黄雯做会议报告。王际睿当选为第十五届大会（日本筑波，2022）主席。
感谢课题组及小麦研究所、四川农业大学、四川省农科院等老师、同学们对会议的支持，同时感谢罗氏、博奥晶典、库蓝科技、瀚辰光翼、慧诺瑞德、肖邦技术等公司的协助。
20181117：科技部完成国家973计划项目“芽麦对品质的影响及抗穗发芽小麦材料全基因组选育”验收。
20180822：周勇等论文“白皮小麦抗穗发芽资源评价及抗性候选位点关联分析”在麦类作物学报发表。
20180121：周勇等论文“Uncovering the Dispersion History, Adaptive Evolution and Selection of Wheat in China”在Plant Biotechnology Journal发表。
20171214-15：王际睿、程梦萍、陈中旭参加2017年度国家超级计算广州中心“天河二号”超算应用大会，“作物基因组大数据分析”工作获得2017年度优秀应用奖。
20170909-22：王际睿、陈中旭参加国际种子科学大会（Monterey, CA），并做“Characterization of pre-harvest sprouting resistance genes in a large germplasm collection of Chinese wheat landraces & Aegilops tauschii”报告；访问UC Davis。
20170822：郭晓江参加第18届全国植物基因组学大会（兰州），获得优秀墙报奖。
20170715-29：王际睿参加“中-澳青年科学家交流项目”（Australia China Young Scientists Exchange Program）访问澳大利亚CSIRO_Canberra, CSIRO_Brisbane, University of Queensland, Murdoch University, University of West Australia，进行学术交流与报告。
20170702：郭晓江论文“Global identification, structural analysis and expression characterization of bHLH transcription factors in wheat”在BMC Plant Biology发表。
20170610：陈学伟团队完成的“A natural allele of a transcription factor in rice confers broad-spectrum blast"在Cell发表。王际睿、程梦萍、陈中旭进行了该论文大数据分析部分工作。
20170514：王际睿参加第五届全国种子科学与技术学术研讨会（桂林）并做学术报告。
20170429：周勇等论文“Genome-Wide Association Study for Pre-harvest Sprouting Resistance in a Large Germplasm Collection of Chinese Wheat Landraces”在Frontiers in Plant Science发表。
20170228：陈真勇等论文“Identification and positional distribution analysis of transcription factor binding sites for genes from the wheat fl-cDNA sequnces”在Bioscience, Biotechnology, and Biochemistry发表。
20170126：周科等论文“Sequence analysis and expression profiles of TaABI5, a pre-harvest sprouting resistance gene in wheat” 在Gene & Geno发表。
20161227：刘宇娇等论文“Conferring resistance to pre-harvest sprouting in durum wheat by a QTL identified in Triticum spelta”在Euphytica发表。
20160918：王际睿、周勇参加第13届国际谷物穗发芽会议（澳大利亚-佩斯）并分别做主题报告，同时成功申请承办下一届会议。
20160814：王际睿、刘宇娇、谭超参加第7届世界作物学大会（北京），期间王际睿做主题报告。
20160315：陈中旭等论文“SNP mining in functional genes from nonmodel species by next-generation sequencing: ... in wheat”在BMRI发表。
20151112：王际睿、杨剑、周勇参加第7届全国小麦遗传育种研讨会（河南-郑州），周勇获得优秀博士研究生墙报奖。
20150818：王际睿、杨剑、周勇参加第6届中国小麦基因组与分子育种大会（陕西-杨凌），王际睿做大会特邀报告。
20150302：陈真勇、郭晓江等论文“Genome-wide characterization of developmental stage- and tissue-specific transcription factors in wheat”在BMC Genomics发表。
20140408：杨剑等论文“Molecular characterization of high pI α-amylase and its expression QTL analysis in synthetic wheat RILs”被Molecular Breeding接收。
Research Assistant
Zao-Xia Wang(王早霞), MSc, South West University (2013-2016)
Maolian Li(李茂莲), MSc, South West University (2016-now)
Xi Long(龙茜), MSc, Jilin Agricultural University (2013-2014)
Jing-Qiang Li(李净琼), MSc, Sichuan Agricultural University (2009-2013)
Mengping Cheng(程梦萍), BSc, Chengdu Neusoft University (2015-now)
Ph.D. Students
Zhen-Yong Chen(陈真勇) (2013-2015)
Jian Yang(杨剑) (2012-2016)
Yong Zhou(周勇) (2012-2017)
Yu-jiao Liu(刘宇娇) (2014-2018; 联合培养: 2014.09-2015.09, AAFC-Ottawa加拿大)
Zhong-xu Chen(陈中旭) (2015-now; 联合培养: 2016.04-2017.09, UC Davis美国)
Lixuan Gui(桂李暄) (2016-now)
Qin Zhang(张琴) (2016-now; 联合培养: 2018.11-2021.2, CSIRO_Canberra澳大利亚)
Xiao-Jiang Guo(郭晓江) (2017-now; 联合培养: 2018.12-now, UC Davis美国)
Iqbal Hussain (2017-2018)
Tang Hao (唐豪) (2015-now)
Tan Chao (谭超) (2015-now)
Jin Lang(郎静)(2016-now)
Yu-Xin Fu(符语昕)(2017-now)
MSc Students
Kun Liu(刘昆) (2011-2014)
Zhong-xu Chen(陈中旭) (2012-2015)
Qin Zhang(张琴) (2013-2016)
Song Zhu(朱松) (2013-2016)
Xiao-Jiang Guo(郭晓江) (2014-2017)
Kwame Obeng Dankwa (2015-2017)
Chunsheng Xiao(肖春生) (2016-2020; 2019.11-2020.04, Murdoch澳大利亚)
Lisheng Yang(杨力生)(2016-2019)
Wen Huang(黄雯)(2017-2020)
Xiangxiang Wang(王祥向)(2017-2020)
Min Deng(邓敏)(2017-2020)
Undergraduate Students
2016: 郭世宽, 曾小玉, 李晶, 端木笑盈, 王冉, 张承碧, 李承志
2015: Xinyuan Ma(马欣源)
2014: Yu-Xin Fu(符语昕)
2013: Rui-wen Mao(毛瑞文), Jiao Xie(谢娇)
2012: Can Yu(余璨), Wen-Shuai Chen(陈文帅), Chun-sheng Xiao(肖春生), Li-na Chen(陈丽娜)
2011: Ke Zhou(周科), Jie Duan(段杰), Jing Lang(郎静)
Location：