Evolutionary Dynamics of the Pgk1 Gene in the Polyploid Genus Kengyilia (Triticeae: Poaceae) and Its Diploid Relatives

Click here to read fulltext

Xing Fan^1,2#, Li-Na Sha^1#, Jian Zeng³, Hou-Yang Kang¹, Hai-Qin Zhang¹, Xiao-Li Wang⁴, Li Zhang⁴, Rui-Wu Yang⁴, Chun-Bang Ding⁴, You-Liang Zheng^1,2, Yong-Hong Zhou^1,2*

1 Triticeae Research Institute, Sichuan Agricultural University, Sichuan, People's Republic of China, 2 Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Sichuan, People's Republic of China, 3 College of Resources and Environment, Sichuan Agricultural University, Sichuan, People's Republic of China, 4 Department of Biology and Science, Sichuan Agricultural University, Sichuan, People's Republic of China

* E-mail: zhouyh@sicau.edu.cn
# These authors contributed equally to this work.

Abstract

The level and pattern of nucleotide variation in duplicate gene provide important information on the evolutionary history of polyploids and divergent process between homoeologous loci within lineages. Kengyilia is a group of allohexaploid species with the StYP genomic constitutions in the wheat tribe. To investigate the evolutionary dynamics of the Pgk1 gene in Kengyilia and its diploid relatives, three copies of Pgk1 homoeologues were isolated from all sampled hexaploid Kengyilia species and analyzed with the Pgk1 sequences from 47 diploid taxa representing 18 basic genomes in Triticeae. Sequence diversity patterns and genealogical analysis suggested that (1) Kengyilia species from the Central Asia and the Qinghai-Tibetan plateau have independent origins with geographically differentiated P genome donors and diverged levels of nucleotide diversity at Pgk1 locus; (2) a relatively long-time sweep event has allowed the Pgk1 gene within Agropyron to adapt to cold climate triggered by the recent uplifts of the Qinghai-Tibetan Plateau; (3) sweep event and population expansion might result in the difference in the dN/dS value of the Pgk1 gene in allopatric Agropyron populations, and this difference may be genetically transmitted to Kengyilia lineages via independent polyploidization events; (4) an 83 bp MITE element insertion has shaped the Pgk1 loci in the P genome lineage with different geographical regions; (5) the St and P genomes in Kengyilia were donated by Pseudoroegneria and Agropyron, respectively, and the Y genome is closely related to the Xp genome of Peridictyon sanctum. The interplay of evolutionary forces involving diverged natural selection, population expansion, and transposable events in geographically differentiated P genome donors could attribute to geographical differentiation of Kengyilia species via independent origins.