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Expression profiling identifies differentially expressed genes associated with the fusarium head blight resistance QTL 2DL from the wheat variety Wuhan-1

Counts:DateTime:2016-01-07 17:38:38 Source: Wheat Research Institute

Physiological and Molecular Plant Pathology, Volume 90, April 2015, Pages 1–11


XiangYu Long, Margaret Balcerzak, Sigrun Gulden, Wenguang Cao, George Fedaka, Yu-Ming Wei, You-Liang Zheng, Daryl Somers, Thérèse Ouellet


(1)Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, 960 Carling Ave, Ottawa, ON K1A 0C6, Canada
(2)Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737, PR China
(3)Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, Sichuan 611130, PR China
(4)Vineland Research and Innovations Centre Inc., 4890 Victoria Avenue North, Box 4000, Vineland Station, ON L0R 2E0, Canada

Abstract
To better understand the contribution of the quantitative fusarium head blight (FHB) resistance locus, 2DL QTL from Wuhan-1, we have characterized two pairs of sister lines carrying either the 2DL QTL or no known QTLs. In wheat spikes, our visual and microscopic observations suggested that the 2DL QTL suppress fungal infection in inoculated spikelets and reduce spread from spikelets to rachis. Transcriptome profiling of infected spikelets showed changes in expression for 487 probe sets between the 2DL-carrying and the null lines. Of 27 transcripts selected for validation, eight candidate genes with similar expression profiles between the two pair of lines were identified.